Journal: Current Research in Neurobiology

Article Title: Gene delivery to neurons in the auditory brainstem of barn owls using standard recombinant adeno-associated virus vectors

doi: 10.1016/j.crneur.2020.100001

Figure Lengend Snippet: Examples for the expression of serotypes rAAV2/1 and rAAV2/5 in nucleus magnocellularis . A–D: Expression of GFP, indicating rAAV2/1, in NM cell bodies and fibers, three weeks after injection (case #13 in Table 1 ). E–H: Expression of tdTomato, indicating rAAV2/5, in NM cell bodies and fibers, also three weeks after injection (case #14 in Table 1 ). Two examples of labeled cell bodies are highlighted by white arrowheads. Note that in each row (A–C and E–G), the first three panels illustrate identical image frames but separately for 3 fluorescence channels; D and H show the corresponding merged images. Scale bars 50 ​μm. For further information about injection site, volume and expression time, see Table 1 .

Article Snippet: For fluorescent detection and imaging, a Nikon epifluorescence microscope (Nikon Eclipse Ni-E and the associated Nikon imaging software NIS Elements AR.4.30.02 64-Bit), as well as a fluorescence slide scanner (Axio Scan Z1, Zeiss, and the associated software ZEISS Efficient Navigation ZEN 2.6 blue edition) were used.

Techniques: Expressing, Injection, Labeling, Fluorescence

Journal: Current Research in Neurobiology

Article Title: Gene delivery to neurons in the auditory brainstem of barn owls using standard recombinant adeno-associated virus vectors

doi: 10.1016/j.crneur.2020.100001

Figure Lengend Snippet: Examples for the expression of serotypes rAAV2/1 and rAAV2/5 in nucleus laminaris . A–D: Expression of GFP, indicating rAAV2/1, in NM projection fibers ramifying within NL, three weeks after injection (case #13 in Table 1 ). E–H: Expression of tdTomato, indicating rAAV2/5, expressed in NM projection fibers ramifying and terminating within NL, also three weeks after injection (case #14 in Table 1 ). One example each of a labeled fiber (B) and terminals surrounding a (non-fluorescent) cell body (G) are highlighted by gray arrowheads, and also shown in higher-magnification insets. As in Fig. 2 , labeled fibers mostly appear as short fragments because their course was oblique to the sectioning plane. Note also that in each row (A–C and E–G), the first three panels illustrate identical image frames but separately for 3 fluorescence channels; D and H show the corresponding merged images. Scale bars 50 ​μm. For detailed information about injection site, volume and expression time, see Table 1 .

Article Snippet: For fluorescent detection and imaging, a Nikon epifluorescence microscope (Nikon Eclipse Ni-E and the associated Nikon imaging software NIS Elements AR.4.30.02 64-Bit), as well as a fluorescence slide scanner (Axio Scan Z1, Zeiss, and the associated software ZEISS Efficient Navigation ZEN 2.6 blue edition) were used.

Techniques: Expressing, Injection, Labeling, Fluorescence